IN VIVO EVALUATION / IN VITRO EVALUATION
Atlangram’s researchers involved in animal experiment have completed training qualifications. All animals (mice, rabbits) used in research are kept in University-inspected and approved housing sites (UTE, Experimental Therapeutic Unit). All authorizations to conduct experiments on animals are up-to-date and have been obtained from the competent authority (UTE, Agreement n° A44-279).
Atlangram follows the national laws and the Directive 2010/63/EU on the protection of animals used for scientific purposes. Atlangram is aware of the opinion given to the European Commission by the “European Group on Ethics in Science and New Technologies” on “Ethical aspects of genetic modification of animals” (Number 7, 21st of May, 1996). The sufferance of all animal used in this project will be kept at a minimum. In establishing an experimental protocol, the concept of the “3Rs” (Replacement, Reduction and Refinement) are followed as close as possible. Care is taken to ensure that the studies are directly relevant to the project and that a minimum number of animals are used for the maximum return of information.
IN VIVO EVALUATION
IN VITRO EVALUATION
E. faecalis, S. aureus, P. aeruginosa, E. coli, K. pneumoniae, Enterobacter, Serratia, Corynebacterium
S. aureus, K. pneumoniae, P. aeruginosa, A. baumannii
Gram +, Gram –.
Gram +, Gram –.
Thigh Infection Model:
S. aureus, E. coli, K. pneumoniae
Urinary Tract Infection (UTI) Model:
Traumatic Brain Injury (TBI) Model:
Catheter-Associated Biofilm Model:
- Determination of PK/PD parameters [Cmax, Tmax, half-life, AUC), % T > MIC etc.]
- Survival and CFU endpoints (fluids, lung, spleen, liver, kidneys)
- Detection of the emergence of resistant variants during therapy
- Survival curves and ED50 determination (peritonitis model)
- Surrogate markers of lung damage (pneumonia model: myeloperoxydase activity, histology, immunohistochemistry, pro-inflammatory cytokines, endothelial permeability)
- Pro and Anti-inflammatory cytokines quantification (ELISA)
- MICs, MBCs, PAE, Killing curves
- Determination of mutation frequencies
- Combinations studies: FIC index, time-kill studies, checkerboard (classic or dynamic approach)
- Studies with carriers of antibiotics (nanoparticles, biomaterials...)
- Ex vivo activity of antibiotics in complex media type fibrin clots